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ctsR [2017-8-11 16:0:3]
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ctsR [2017-8-11 16:0:3]

transcription repressor of class III heat shock genes ([gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC] operon, [gene|8C5B14FE5E03427F9A598C75D4081FA0D6696299|clpE], [gene|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP])
locus
BSU00830
pI
9.26
mw
17.60 kDa
protein length
154 aa Sequence Blast
gene length
462 bp Sequence Blast
function
regulation of protein degradation
product
transcription repressor
essential
no
synonyms
yacG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • Gene

    Coordinates
    101,449 → 101,913

    The protein

    Protein family

  • ctsR family (according to Swiss-Prot)

    • Modification

  • phosphorylated on Arg-55 (phosphorylation serves as tag for degradation by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP]) [Pubmed|27749819]
  • phosphorylation of a tyrosine residue by [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|McsB] [Pubmed|14984053]
  • recently, it was reported that CtsR is phosphorylated by [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|McsB] on Arg-62 rather than on a tyrosine residue [Pubmed|19498169]
  • in addition, Arg-15 was reported to be a phosphorylation site [Pubmed|22517742]

    • Effectors of protein activity

  • CtsR is inactivated by heat, heat sensing requires Gly-64 [Pubmed|20852588]
  • non-phosphorylated [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|McsB] targets CtsR for degradation [Pubmed|20852588]
  • regulated proteolysis by [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP]/[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC] [Pubmed|11179229,16163393,17380125]

    • Structure

  • [PDB|3H0D] (complex with a 26bp DNA duplex, from ''Geobacillus stearothermophilus'') [Pubmed|19498169]

    • additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [pubmed|17981983]

    • Expression and Regulation

    Operons

    genes
    [gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]-[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]-[gene|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]-[gene|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]-[gene|151F226370D225776F3FE7EA4901485095F1AC45|radA]-[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]
    description
    [Pubmed|8793870]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|17434969], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8793870], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [pubmed|8793870] [pubmed|11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

    • regulatory mechanism

  • [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]: repression, [pubmed|9987115,11179229,16163393,17380125], in [regulon|908DB17A39D518E84977250C55825E77FA02E391|CtsR regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [pubmed|30962353], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

    • regulation

  • expressed during germination and spore outgrowth [Pubmed|24244006]
  • induction during diamide stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]) [pubmed|30962353]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B928 (yacG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1927 NBRP B. subtilis, Japan]
  • ''ctsR::aphA3'' availbale in [SW|Ulf Gerth]'s lab
  • BKE00830 (''[gene|908DB17A39D518E84977250C55825E77FA02E391|ctsR]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]

    • Expression vector

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • available in [SW|Ulf Gerth]'s lab

    • References

    Reviews

  • 27518094,28402413,28748186

    • Original Publications

  • 14984053,21078442,23375660,27749819